Soybean’s protein concentration and amino acid profile vary geographically due to genotype and environment. We used 1817 on-farm grain samples from the United States, Brazil, and the Southern Cone to assess soybean protein variability in the Western Hemisphere, which accounts for 85% of global production. Our analysis found consistent spatial patterns for protein concentration and amino acid composition, with differences in protein concentration of up to 5.4% among regions. While there was a negative correlation between protein concentration and the abundance of critical amino acids (lysine, cysteine, threonine, methionine, and tryptophane), their amount per ton of grain increased with protein concentration.