Two experiments were conducted to test the hypothesis that nitrogen-corrected true metabolizable energy (TMEn) and standardized amino acid (AA) digestibility are not different in 3 new lines of soybeans with increased crude protein (CP) and increased sulfur AA (SAA) compared with conventional soybeans fed to chickens. The 3 new cross lines of soybeans were developed by crossing a high SAA (i.e. ‘CS’) with a high CP (i.e. ‘Lee 5’) soybean lines. Six soybeans (the 2 parent CS and Lee 5 lines, the 3 cross lines, and Maverick conventional) were evaluated. The 6 soybeans were autoclaved for 25 min to reduce antinutritional factors. The Maverick conventional contained 35.5% CP (1.11% SAA) and 20.1% crude fat. The CS contained similar CP and fat levels, but greater SAA content (1.42% SAA) compared with conventional soybeans. The Lee 5 contained 44.7% CP (1.28% SAA) and 13.3% fat. Thus, as expected, the new cross lines of soybeans had greater CP and SAA content, but lower crude fat levels compared with conventional soybeans. All AA levels were increased in the 3 cross lines, with the largest increase being for Cys which increased from 0.58% in the conventional to 0.82-0.99% in the 3 cross lines. In Experiments 1 and 2, two precision-fed rooster assays were conducted to determine TMEn and standardized AA digestibility in the 6 soybeans using conventional and cecectomized roosters, respectively. For each experiment, 30 individually caged White Leghorn roosters (1 year old) were fasted for 26 h and then crop intubated with 25 g of 1 of the 6 soybeans with a total of 5 roosters per soybean sample. Excreta were then collected quantitatively for 48 h post-feeding. Excreta samples were analyzed for gross energy, nitrogen, and AA. Standardized AA digestibility values were obtained from apparent AA digestibility then corrected for endogenous AA losses. Data were analyzed as a one-way ANOVA for a completely randomized design. Differences among treatments were considered significant at P < 0.05. Results from Experiment 1 yielded TMEn values between 2.921 and 3.651 kcal/g DM with the values being greater (P < 0.05) for conventional than the Lee 5 and 3 cross soybeans. These results may be explained by the higher fat content in the conventional compared with the other soybean lines. Results from Experiment 2 showed no differences (P > 0.05) in standardized digestibility coefficients for AA among soybeans.